| Adams | Adams County Health & Human Services | Friendship | Southern |
| Ashland | Ashland County Health & Human Services | Ashland | Northern |
| Barron | Barron County Department of Health & Human Services | Barron | Western |
| Bayfield | Bayfield County Health Department | Washburn | Northern |
| Brown | Brown County Health & Human Services Department | Green Bay | Northeastern |
| Brown | DePere Department of Public Health | DePere | Northeastern |
| Buffalo | Buffalo County Health & Human Services Department | Alma | Western |
| Burnett | Burnett County Department of Health & Human Services | Siren | Western |
| Calumet | Appleton City Health Department | Appleton | Northeastern |
| Calumet | Calumet County Health & Human Services | Chilton | Northeastern |
| Calumet | City of Menasha Health Department | Menasha | Northeastern |
| Chippewa | Chippewa County Department of Public Health | Chippewa Falls | Western |
| Clark | Clark County Health Department | Neillsville | Western |
| Columbia | Columbia County Health & Human Services | Portage | Southern |
| Crawford | Crawford County Health & Human Services | Prairie du Chien | Southern |
| Dane | Public Health - Madison & Dane County | Madison | Southern |
| Dodge | Dodge County Human Services & Health Department | Juneau | Southern |
| Dodge | Watertown Department of Public Health | Watertown | Southern |
| Door | Door County Department of Health & Human Services | Sturgeon Bay | Northeastern |
| Douglas | Douglas County Department of Health & Human Services | Superior | Western |
| Dunn | Dunn County Health Department | Menomonie | Western |
| Eau Claire | Eau Claire City-County Health Department | Eau Claire | Western |
| Florence | Florence County Health Department | Florence | Northern |
| Forest | Forest County Health Department | Crandon | Northern |
| Fond du Lac | Fond du Lac County Health Department | Fond du Lac | Northeastern |
| Grant | Grant County Health Department | Lancaster | Southern |
| Green | Green County Public Health | Monroe | Southern |
| Green Lake | Green Lake County Department of Health & Human Services | Green Lake | Northeastern |
| Iowa | Iowa County Health Department | Dodgeville | Southern |
| Iron | Iron County Health Department | Hurley | Northern |
| Jackson | Jackson County Public Health Department | Black River Falls | Western |
| Jefferson | Jefferson County Health Department | Jefferson | Southern |
| Jefferson | Watertown Department of Public Health | Watertown | Southern |
| Juneau | Juneau County Health Department | Mauston | Southern |
| Kenosha | Kenosha County Division of Health | Kenosha | Southeastern |
| Kewaunee | Kewaunee County Public Health Department | Kewaunee | Northeastern |
| La Crosse | La Crosse County Health Department | La Crosse | Western |
| Lafayette | Lafayette County Health Department | Darlington | Southern |
| Langlade | Langlade County Health Department | Antigo | Northern |
| Lincoln | Lincoln County Health Department | Merrill | Northern |
| Manitowoc | Manitowoc County Health Department | Manitowoc | Northeastern |
| Marathon | Marathon County Health Department | Wausau | Northern |
| Marinette | Marinette County Health & Human Services | Marinette | Northeastern |
| Marquette | Marquette County Health Department | Montello | Northeastern |
| Menominee | Shawano-Menominee Counties Health Department | Shawano | Northeastern |
| Milwaukee | Cudahy Health Department | Cudahy | Southeastern |
| Milwaukee | Franklin Health Department | Franklin | Southeastern |
| Milwaukee | Greendale Health Department | Greendale | Southeastern |
| Milwaukee | Hales Corners Health Department | Hales Corners | Southeastern |
| Milwaukee | Milwaukee City Health Department | Milwaukee | Southeastern |
| Milwaukee | North Shore Health Department | Brown Deer | Southeastern |
| Milwaukee | Oak Creek Health Department | Oak Creek | Southeastern |
| Milwaukee | South Milwaukee/St. Francis Health Department | South Milwaukee | Southeastern |
| Milwaukee | Southwest Suburban Health Department | West Allis | Southeastern |
| Milwaukee | Wauwatosa Health Department | Wauwatosa | Southeastern |
| Monroe | Monroe County Health Department | Sparta | Western |
| Oconto | Oconto County Health & Human Services Department, Public Health Division | Oconto | Northeastern |
| Oneida | Oneida County Health Department | Rhinelander | Northern |
| Outagamie | Appleton City Health Department | Appleton | Northeastern |
| Outagamie | Outagamie County Health & Human Services | Appleton | Northeastern |
| Ozaukee | Washington Ozaukee Public Health Department | Port Washington | Southeastern |
| Pepin | Pepin County Health Department | Durand | Western |
| Pierce | Pierce County Public Health Department | Ellsworth | Western |
| Polk | Polk County Health Department | Balsam Lake | Western |
| Portage | Portage County Health & Human Services | Stevens Point | Northern |
| Price | Price County Health & Human Services | Phillips | Northern |
| Racine | City of Racine Public Health Department | Racine | Southeastern |
| Racine | Racine County Public Health Division | Sturtevant | Southeastern |
| Richland | Richland County Health & Human Services | Richland Center | Southern |
| Rock | Rock County Public Health Department | Janesville | Southern |
| Rusk | Rusk County Health & Human Services | Ladysmith | Western |
| Sauk | Sauk County Health Department | Baraboo | Southern |
| Sawyer | Sawyer County Health & Human Services | Hayward | Northern |
| Shawano | Shawano-Menominee Counties Health Department | Shawano | Northeastern |
| Sheboygan | Sheboygan County Health & Human Services | Sheboygan | Northeastern |
| St. Croix | St. Croix County Health & Human Services | New Richmond | Western |
| Taylor | Taylor County Health Department | Medford | Northern |
| Trempealeau | Trempealeau County Health Department | Whitehall | Western |
| Vernon | Vernon County Public Health Department | Viroqua | Southern |
| Vilas | Vilas County Public Health Department | Eagle River | Northern |
| Walworth | Walworth County Department of Health & Human Services | Elkhorn | Southeastern |
| Washburn | Washburn County Health Department | Shell Lake | Western |
| Washington | Washington Ozaukee Public Health Department | Port Washington | Southeastern |
| Waukesha | Waukesha County Department of Health & Human Services | Waukesha | Southeastern |
| Waupaca | Waupaca County Department of Public Health | Waupaca | Northeastern |
| Waushara | Waushara County Health Department | Wautoma | Northeastern |
| Winnebago | Appleton City Health Department | Appleton | Northeastern |
| Winnebago | City of Menasha Health Department | Menasha | Northeastern |
| Winnebago | Winnebago County Health Department | Oshkosh | Northeastern |
| Wood | Wood County Health Department | Wisconsin Rapids | Northern |